MTRR Knockout cell line (HepG2)

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location： Home > Products > KO Cell Lines > MTRR Knockout cell line (HepG2)

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MTRR Knockout cell line (HepG2) Order

MTRR Knockout cell line (HepG2)
Catalog#
YKO-H826
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MTRR Knockout cell line (HepG2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MTRR Knockout cell line (HepG2)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
MTRR
Gene ID
4552
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10% FBS
Target region
Knockout region: Exon 3 (based on transcript MTRR-202)
Validation: PCR+Sanger Sequencing
MTRR Knockout cell line (HepG2) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:Hep G2		 STR Info (Cell bank)
Cell Line:GS-HepG2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 913913
D7S820 10101010
D16S539 12121212
VWA 17171717
TH01 9999
AMEL XYXY
TPOX 8989
CSF1PO 10111011
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MTRR gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MTRR gene knockout MTRR Knockout cell line (HepG2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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